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8  structures 176  species 0  interactions 497  sequences 7  architectures

Family: Arabinose_trans (PF04602)

Summary: Mycobacterial cell wall arabinan synthesis protein

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This is the Wikipedia entry entitled "Arabinosyltransferase". More...

Arabinosyltransferase Edit Wikipedia article

Arabinosyltransferase
Identifiers
Symbol Arabinose_trans
Pfam PF04602
Pfam clan CL0111
InterPro IPR007680
CAZy GT53

In molecular biology, an arabinosyltransferase is a transferase enzyme acting upon arabinose. This enzyme is involved in polymerisation of arabinogalactan (an essential component of the mycobacterial cell wall). In terms of mycobacteria, the more precise term is arabinofuranosyltransferase, since the arabinose residues occur only in a furanose form.

This enzyme has important clinical applications as it is believed to be the target of the antimycobacterial drug Ethambutol.[1]

References[edit]

  1. ^ Belanger AE, Besra GS, Ford ME, Mikusova K, Belisle JT, Brennan PJ, Inamine JM (October 1996). "The embAB genes of Mycobacterium avium encode an arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol". Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11919–24. doi:10.1073/pnas.93.21.11919. PMC 38159. PMID 8876238. 

This article incorporates text from the public domain Pfam and InterPro IPR007680

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Mycobacterial cell wall arabinan synthesis protein Provide feedback

Arabinosyltransferase is involved in arabinogalactan (AG) biosynthesis pathway in mycobacteria. AG is a component of the macromolecular assembly of the mycolyl-AG-peptidoglycan complex of the cell wall. This enzyme has important clinical applications as it is believed to be the target of the antimycobacterial drug Ethambutol [1].

Literature references

  1. Belanger AE, Besra GS, Ford ME, Mikusova K, Belisle JT, Brennan PJ, Inamine JM; , Proc Natl Acad Sci U S A 1996;93:11919-11924.: The embAB genes of Mycobacterium avium encode an arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol. PUBMED:8876238 EPMC:8876238


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007680

Arabinosyltransferases EmbA, EmbB and EmbC are known to be involved in arabinogalactan (AG) and lipoarabinomannan (LAM) biosynthesis in mycobacteria. Synthesis of LAM, but not AG, ceases after inactivation of EmbC, while the arabinan of AG is affected by EmbA or EmbB disruption [PUBMED:14507364]. The arabinans of the mycobacterial cell wall are key structural and immunological polymers.

These enzymes could have important clinical applications as they are believed to be the target of the antimycobacterial drug Ethambutol [PUBMED:8876238].

Gene Ontology

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Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(7)
Full
(497)
Representative proteomes NCBI
(457)
Meta
(0)
RP15
(15)
RP35
(61)
RP55
(81)
RP75
(90)
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Format an alignment

  Seed
(7)
Full
(497)
Representative proteomes NCBI
(457)
Meta
(0)
RP15
(15)
RP35
(61)
RP55
(81)
RP75
(90)
Alignment:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(7)
Full
(497)
Representative proteomes NCBI
(457)
Meta
(0)
RP15
(15)
RP35
(61)
RP55
(81)
RP75
(90)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_4670 (release 7.5)
Previous IDs: arab_transf;
Type: Family
Author: Waterfield DI, Finn RD
Number in seed: 7
Number in full: 497
Average length of the domain: 565.40 aa
Average identity of full alignment: 47 %
Average coverage of the sequence by the domain: 61.09 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 28.0 27.9
Noise cut-off 17.9 22.2
Model length: 657
Family (HMM) version: 7
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Arabinose_trans domain has been found. There are 8 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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