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0  structures 135  species 0  interactions 347  sequences 7  architectures

Family: Glyco_hydro_100 (PF12899)

Summary: Alkaline and neutral invertase

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This is the Wikipedia entry entitled "Glycoside hydrolase family 100". More...

Glycoside hydrolase family 100 Edit Wikipedia article

Alkaline and neutral invertase
Identifiers
Symbol Glyco_hydro_100
Pfam PF12899
Pfam clan CL0059
CAZy GH100

In molecular biology, glycoside hydrolase family 100 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]

Glycoside hydrolase family 100 CAZY GH_100 includes enzymes with invertase activity EC 3.2.1.26.[6][7]

References[edit]

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Alkaline and neutral invertase Provide feedback

This is a family of bacterial and plant alkaline and neutral invertases, EC:3.2.1.26, previously known as Invertase_neut PF04853.

Literature references

  1. Lee HS, Sturm A; , Plant Physiol 1996;112:1513-1522.: Purification and characterization of neutral and alkaline invertase from carrot. PUBMED:8972597 EPMC:8972597

  2. Sturm A;, Plant Physiol. 1999;121:1-8.: Invertases. Primary structures, functions, and roles in plant development and sucrose partitioning. PUBMED:10482654 EPMC:10482654


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR024746

O-Glycosyl hydrolases (EC) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PUBMED:7624375, PUBMED:8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycosyl hydrolase family 100 includes enzymes with invertase activity [PUBMED:8972597, PUBMED:10482654].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(20)
Full
(347)
Representative proteomes NCBI
(342)
Meta
(258)
RP15
(28)
RP35
(99)
RP55
(142)
RP75
(171)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(20)
Full
(347)
Representative proteomes NCBI
(342)
Meta
(258)
RP15
(28)
RP35
(99)
RP55
(142)
RP75
(171)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(20)
Full
(347)
Representative proteomes NCBI
(342)
Meta
(258)
RP15
(28)
RP35
(99)
RP55
(142)
RP75
(171)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: manual
Previous IDs: none
Type: Domain
Author: Coggill P
Number in seed: 20
Number in full: 347
Average length of the domain: 358.00 aa
Average identity of full alignment: 51 %
Average coverage of the sequence by the domain: 75.19 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.6 23.6
Trusted cut-off 23.6 23.6
Noise cut-off 23.5 23.4
Model length: 436
Family (HMM) version: 2
Download: download the raw HMM for this family

Species distribution

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