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0  structures 236  species 0  interactions 316  sequences 9  architectures

Family: HECT_2 (PF09814)

Summary: HECT-like Ubiquitin-conjugating enzyme (E2)-binding

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This is the Wikipedia entry entitled "HECT domain". More...

HECT domain Edit Wikipedia article

HECT-domain (ubiquitin-transferase)
PDB 1d5f EBI.jpg
structure of an e6ap-ubch7 complex: insights into the ubiquitination pathway
Identifiers
Symbol HECT
Pfam PF00632
InterPro IPR000569
SCOP 1d5f
SUPERFAMILY 1d5f

In molecular biology, the HECT domain is a protein domain found in ubiquitin-protein ligases. The name HECT comes from 'Homologous to the E6-AP Carboxyl Terminus'.[1] Proteins containing this domain at the C terminus include ubiquitin-protein ligase, which regulates ubiquitination of CDC25. Ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester, and then directly transfers the ubiquitin to targeted substrates. A cysteine residue is required for ubiquitin-thiolester formation. Human thyroid receptor interacting protein 12 (TRIP12), which also contains this domain, is a component of an ATP-dependent multisubunit protein that interacts with the ligand binding domain of the thyroid hormone receptor. It could be an E3 ubiquitin-protein ligase. Human ubiquitin-protein ligase E3A interacts with the E6 protein of the cancer-associated Human papillomavirus type 16 and Human papillomavirus type 18. The E6/E6-AP complex binds to and targets the P53 tumour-suppressor protein for ubiquitin-mediated proteolysis.


References[edit]

  1. ^ Huibregtse JM, Scheffner M, Beaudenon S, Howley PM (March 1995). "A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase". Proc. Natl. Acad. Sci. U.S.A. 92 (7): 2563–7. doi:10.1073/pnas.92.7.2563. PMC 42258. PMID 7708685. 

This article incorporates text from the public domain Pfam and InterPro IPR000569

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HECT-like Ubiquitin-conjugating enzyme (E2)-binding Provide feedback

HECT_2 is a family of UbcH10-binding proteins.

Literature references

  1. Kobirumaki F, Miyauchi Y, Fukami K, Tanaka H;, J Biochem. 2005;137:133-139.: A novel UbcH10-binding protein facilitates the ubiquitinylation of cyclin B in vitro. PUBMED:15749827 EPMC:15749827


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019193

This entry consists of E3 ubiquitin-protein ligases which accept ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfer it to substrates, generally promoting their degradation by the proteasome [PUBMED:15749827].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Hect (CL0552), which contains the following 2 members:

HECT HECT_2

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(28)
Full
(316)
Representative proteomes NCBI
(300)
Meta
(2)
RP15
(65)
RP35
(116)
RP55
(185)
RP75
(227)
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Format an alignment

  Seed
(28)
Full
(316)
Representative proteomes NCBI
(300)
Meta
(2)
RP15
(65)
RP35
(116)
RP55
(185)
RP75
(227)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(28)
Full
(316)
Representative proteomes NCBI
(300)
Meta
(2)
RP15
(65)
RP35
(116)
RP55
(185)
RP75
(227)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: KOGs (KOG4784)
Previous IDs: DUF2351;
Type: Family
Author: KOGs, Finn RD, Sammut SJ
Number in seed: 28
Number in full: 316
Average length of the domain: 298.30 aa
Average identity of full alignment: 16 %
Average coverage of the sequence by the domain: 76.93 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 19.0 19.0
Trusted cut-off 19.1 19.2
Noise cut-off 18.9 18.7
Model length: 354
Family (HMM) version: 4
Download: download the raw HMM for this family

Species distribution

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