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0  structures 256  species 0  interactions 291  sequences 7  architectures

Family: Med10 (PF09748)

Summary: Transcription factor subunit Med10 of Mediator complex

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Transcription factor subunit Med10 of Mediator complex Provide feedback

Med10 is one of the protein subunits of the Mediator complex, tethered to Rgr1 protein. The Mediator complex is required for the transcription of most RNA polymerase II (Pol II)-transcribed genes. Med10 specifically mediates basal-level HIS4 transcription via Gcn4, and, additionally, there is a putative requirement for Med10 in Bas2-mediated transcription [1]. Med10 is part of the middle region of Mediator [3].

Literature references

  1. Han SJ, Lee YC, Gim BS, Ryu GH, Park SJ, Lane WS, Kim YJ; , Mol Cell Biol. 1999;19:979-988.: Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation. PUBMED:9891034 EPMC:9891034

  2. Baek HJ, Kang YK, Roeder RG; , J Biol Chem. 2006;281:15172-15181.: Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly. PUBMED:16595664 EPMC:16595664

  3. Bourbon HM, Aguilera A, Ansari AZ, Asturias FJ, Berk AJ, Bjorklund S, Blackwell TK, Borggrefe T, Carey M, Carlson M, Conaway JW, Conaway RC, Emmons SW, Fondell JD, Freedman LP, Fukasawa T, Gustafsson CM, Han M, He X, Herman PK, Hinnebusch AG, Holmberg S, , Mol Cell. 2004;14:553-557.: A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. PUBMED:15175151 EPMC:15175151


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019145

The Mediator complex is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. On recruitment the Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.

The Mediator complex is composed of at least 31 subunits: MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11.

The subunits form at least three structurally distinct submodules. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation.

  • The head module contains: MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22.
  • The middle module contains: MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31. CSE2/MED9 interacts directly with MED4.
  • The tail module contains: MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16.
  • The CDK8 module contains: MED12, MED13, CCNC and CDK8.

Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

Med10 is one of the protein subunits of the Mediator complex, tethered to Med14 (Rgr1) protein. Med10 specifically mediates basal-level HIS4 transcription via Gcn4. In addition, there is a putative requirement for Med10 in Bas2-mediated transcription [PUBMED:9891034].

Gene Ontology

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  Seed
(18)
Full
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Representative proteomes NCBI
(273)
Meta
(0)
RP15
(67)
RP35
(112)
RP55
(168)
RP75
(209)
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  Seed
(18)
Full
(291)
Representative proteomes NCBI
(273)
Meta
(0)
RP15
(67)
RP35
(112)
RP55
(168)
RP75
(209)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

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Pfam alignments:

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Curation and family details

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Curation View help on the curation process

Seed source: KOGs (KOG3046)
Previous IDs: none
Type: Family
Author: KOGs, Finn RD, Coggill P
Number in seed: 18
Number in full: 291
Average length of the domain: 121.30 aa
Average identity of full alignment: 33 %
Average coverage of the sequence by the domain: 74.23 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.5 21.5
Trusted cut-off 21.8 21.9
Noise cut-off 20.3 21.4
Model length: 128
Family (HMM) version: 4
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