Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
0  structures 4727  species 0  interactions 6283  sequences 17  architectures

Family: CTP_transf_1 (PF01148)

Summary: Cytidylyltransferase family

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Phosphatidate cytidylyltransferase". More...

Phosphatidate cytidylyltransferase Edit Wikipedia article

phosphatidate cytidylyltransferase
Identifiers
EC number 2.7.7.41
CAS number 9067-83-8
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO
Cytidylyltransferase family
Identifiers
Symbol CTP_transf_1
Pfam PF01148
InterPro IPR000374
PROSITE PDOC01019

Phosphatidate cytidylyltransferase (EC 2.7.7.41)[1][2][3] (also known as CDP- diacylglycerol synthase) (CDS) is the enzyme that catalyzes the synthesis of CDP-diacylglycerol from cytidine triphosphate and phosphatidate.

CTP + phosphatidate \rightleftharpoons diphosphate + CDP-diacylglycerol

Thus, the two substrates of this enzyme are cytidine triphosphate, or CTP, and phosphatidate, whereas its two products are diphosphate and CDP-diacylglycerol.

CDP-diacylglycerol is an important branch point intermediate in both prokaryotic and eukaryotic organisms. CDS is a membrane-bound enzyme.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is CTP:phosphatidate cytidylyltransferase. Other names in common use include CDP diglyceride pyrophosphorylase, CDP-diacylglycerol synthase, CDP-diacylglyceride synthetase, cytidine diphosphoglyceride pyrophosphorylase, phosphatidate cytidyltransferase, phosphatidic acid cytidylyltransferase, CTP:1,2-diacylglycerophosphate-cytidyl transferase, CTP-diacylglycerol synthetase, DAG synthetase, and CDP-DG. This enzyme participates in glycerophospholipid metabolism and phosphatidylinositol signaling system.

[edit] References

  1. ^ Sparrow CP, Raetz CR (1985). "Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli". J. Biol. Chem. 260 (22): 12084–12091. PMID 2995359. 
  2. ^ Shen H, Heacock PN, Clancey CJ, Dowhan W (1996). "The CDS1 gene encoding CDP-diacylglycerol synthase in Saccharomyces cerevisiae is essential for cell growth". J. Biol. Chem. 271 (2): 789–795. doi:10.1074/jbc.271.2.789. PMID 8557688. 
  3. ^ Kondo H, Goto K, Saito S, Tonosaki A (1997). "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain". J. Biol. Chem. 272 (14): 9503–9509. doi:10.1074/jbc.272.14.9503. PMID 9083091. 
  • Carter JR, Kennedy EP (1966). "Enzymatic synthesis of cytidine diphosphate diglyceride". J. Lipid. Res. 7 (5): 678–83. PMID 4291255. 
  • McCaman RE, Finnerty WR (1968). "Biosynthesis of cytidine diphosphate-diglyceride by a particulate fracgion from Micrococcus cerificans". J. Biol. Chem. 243 (19): 5074–80. PMID 5679981. 
  • Petzold GL, Agranoff BW (1967). "The biosynthesis of cytidine diphosphate diglyceride by embryonic chick brain". J. Biol. Chem. 242 (6): 1187–91. PMID 6067194. 

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Cytidylyltransferase family Provide feedback

The members of this family are integral membrane protein cytidylyltransferases. The family includes phosphatidate cytidylyltransferase EC:2.7.7.41 as well as Sec59 from yeast. Sec59 is a dolichol kinase EC:2.7.1.108.

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000374

Phosphatidate cytidylyltransferase (EC) [PUBMED:2995359, PUBMED:8557688, PUBMED:9083091] (also known as CDP- diacylglycerol synthase) (CDS) is the enzyme that catalyzes the synthesis of CDP-diacylglycerol from CTP and phosphatidate (PA): CTP + phosphatidate = diphosphate + CDP-diacylglycerol CDP-diacylglycerol is an important branch point intermediate in both prokaryotic and eukaryotic organisms. CDS is a membrane-bound enzyme.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan CTPT (CL0234), which contains the following 2 members:

CTP_transf_1 DUF46

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(48)
Full
(6283)
Representative proteomes NCBI
(4702)
Meta
(2431)
RP15
(615)
RP35
(1145)
RP55
(1531)
RP75
(1821)
Jalview View  View  View  View  View  View  View  View 
HTML View    View  View  View  View     
PP/heatmap 1   View  View  View  View     
Pfam viewer View  View             

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(48)
Full
(6283)
Representative proteomes NCBI
(4702)
Meta
(2431)
RP15
(615)
RP35
(1145)
RP55
(1531)
RP75
(1821)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(48)
Full
(6283)
Representative proteomes NCBI
(4702)
Meta
(2431)
RP15
(615)
RP35
(1145)
RP55
(1531)
RP75
(1821)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_921 (release 3.0)
Previous IDs: Cytidylyltrans;
Type: Family
Author: Finn RD, Bateman A
Number in seed: 48
Number in full: 6283
Average length of the domain: 255.40 aa
Average identity of full alignment: 24 %
Average coverage of the sequence by the domain: 86.77 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.6 23.6
Trusted cut-off 23.6 23.6
Noise cut-off 23.5 23.5
Model length: 259
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Show

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.