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8  structures 256  species 0  interactions 627  sequences 21  architectures

Family: GRIP (PF01465)

Summary: GRIP domain

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GRIP domain Edit Wikipedia article

GRIP domain
PDB 1upt EBI.jpg
structure of a complex of the golgin-245 grip domain with arl1
Identifiers
Symbol GRIP
Pfam PF01465
InterPro IPR000237
SCOP 1upt
SUPERFAMILY 1upt

In molecular biology, the GRIP domain is a conserved protein domain. The GRIP (golgin-97, RanBP2alpha, Imh1p and p230/golgin-245) domain is found in many large coiled-coil proteins. It has been shown to be sufficient for targeting to the Golgi. It contains a completely conserved tyrosine residue.[1][2][3]

References[edit]

  1. ^ Munro S, Nichols BJ (April 1999). "The GRIP domain - a novel Golgi-targeting domain found in several coiled-coil proteins". Curr. Biol. 9 (7): 377–80. doi:10.1016/S0960-9822(99)80166-3. PMID 10209120. 
  2. ^ Barr FA (April 1999). "A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins". Curr. Biol. 9 (7): 381–4. doi:10.1016/S0960-9822(99)80167-5. PMID 10209123. 
  3. ^ Kjer-Nielsen L, Teasdale RD, van Vliet C, Gleeson PA (April 1999). "A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins". Curr. Biol. 9 (7): 385–8. doi:10.1016/S0960-9822(99)80168-7. PMID 10209125. 

This article incorporates text from the public domain Pfam and InterPro IPR000237

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

GRIP domain Provide feedback

The GRIP (golgin-97, RanBP2alpha,Imh1p and p230/golgin-245) domain is found in many large coiled-coil proteins. It has been shown to be sufficient for targeting to the Golgi. The GRIP domain contains a completely conserved tyrosine residue. At least some of these domains have been shown to bind to GTPase Arl1, see structures in [4,5].

Literature references

  1. Munro S, Nichols BJ; , Curr Biol 1999;9:377-380.: The GRIP domain - a novel Golgi-targeting domain found in several coiled-coil proteins. PUBMED:10209120 EPMC:10209120

  2. Kjer-Nielsen L, Teasdale RD, van Vliet C, Gleeson PA; , Curr Biol 1999;9:385-388.: A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins. PUBMED:10209125 EPMC:10209125

  3. Barr FA; , Curr Biol 1999;9:381-384.: A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins. PUBMED:10209123 EPMC:10209123

  4. Wu M, Lu L, Hong W, Song H; , Nat Struct Mol Biol. 2004;11:86-94.: Structural basis for recruitment of GRIP domain golgin-245 by small GTPase Arl1. PUBMED:14718928 EPMC:14718928

  5. Panic B, Perisic O, Veprintsev DB, Williams RL, Munro S; , Mol Cell. 2003;12:863-874.: Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus. PUBMED:14580338 EPMC:14580338


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000237

The GRIP (golgin-97, RanBP2alpha,Imh1p and p230/golgin-245) domain [PUBMED:10209120, PUBMED:10209123, PUBMED:10209125] is found in many large coiled-coil proteins. It has been shown to be sufficient for targeting to the Golgi [PUBMED:14580338]. The GRIP domain contains a completely conserved tyrosine residue.

Gene Ontology

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Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

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(44)
Full
(627)
Representative proteomes NCBI
(639)
Meta
(5)
RP15
(146)
RP35
(206)
RP55
(324)
RP75
(415)
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Format an alignment

  Seed
(44)
Full
(627)
Representative proteomes NCBI
(639)
Meta
(5)
RP15
(146)
RP35
(206)
RP55
(324)
RP75
(415)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(44)
Full
(627)
Representative proteomes NCBI
(639)
Meta
(5)
RP15
(146)
RP35
(206)
RP55
(324)
RP75
(415)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Sean Munro
Previous IDs: none
Type: Family
Author: Bateman A
Number in seed: 44
Number in full: 627
Average length of the domain: 44.60 aa
Average identity of full alignment: 31 %
Average coverage of the sequence by the domain: 4.30 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.5 20.5
Trusted cut-off 20.5 20.8
Noise cut-off 19.8 19.7
Model length: 46
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the GRIP domain has been found. There are 8 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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