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0  structures 892  species 0  interactions 1865  sequences 20  architectures

Family: Glyco_transf_25 (PF01755)

Summary: Glycosyltransferase family 25 (LPS biosynthesis protein)

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Glycosyltransferase family 25 (LPS biosynthesis protein) Provide feedback

Members of this family belong to Glycosyltransferase family 25 [1] This is a family of glycosyltransferases involved in lipopolysaccharide (LPS) biosynthesis. These enzymes catalyse the transfer of various sugars onto the growing LPS chain during its biosynthesis.

Literature references

  1. Campbell JA, Davies GJ, Bulone V, Henrissat B; , Biochem J 1997;326:929-939.: A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities PUBMED:9334165 EPMC:9334165

  2. Jennings MP, Hood DW, Peak IR, Virji M, Moxon ER; , Mol Microbiol 1995;18:729-740.: Molecular analysis of a locus for the biosynthesis and phase-variable expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. PUBMED:8817494 EPMC:8817494

  3. Gotschlich EC; , J Exp Med 1994;180:2181-2190.: Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae lipooligosaccharide. PUBMED:7964493 EPMC:7964493

  4. Cope LD, Yogev R, Mertsola J, Latimer JL, Hanson MS, McCracken GH Jr, Hansen EJ; , Mol Microbiol 1991;5:1113-1124.: Molecular cloning of a gene involved in lipooligosaccharide biosynthesis and virulence expression by Haemophilus influenzae type B. PUBMED:1956289 EPMC:1956289


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR002654

The biosynthesis of disaccharides, oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These enzymes catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. A classification of glycosyltransferases using nucleotide diphospho-sugar, nucleotide monophospho-sugar and sugar phosphates (EC) and related proteins into distinct sequence based families has been described [PUBMED:9334165]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. The same three-dimensional fold is expected to occur within each of the families. Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence similarities may have similar 3-D structures and therefore form 'clans'.

Glycosyltransferase family 25 CAZY comprises enzymes with only one known activity; as a lipopolysaccharide biosynthesis protein. These enzymes catalyse the transfer of various sugars onto the growing lipopolysaccharide chain during its biosynthesis [PUBMED:8817494].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(22)
Full
(1865)
Representative proteomes NCBI
(1567)
Meta
(1071)
RP15
(99)
RP35
(205)
RP55
(301)
RP75
(399)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(22)
Full
(1865)
Representative proteomes NCBI
(1567)
Meta
(1071)
RP15
(99)
RP35
(205)
RP55
(301)
RP75
(399)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(22)
Full
(1865)
Representative proteomes NCBI
(1567)
Meta
(1071)
RP15
(99)
RP35
(205)
RP55
(301)
RP75
(399)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1857 (release 4.1)
Previous IDs: LPS_glycoyl_T;
Type: Family
Author: Bashton M, Bateman A
Number in seed: 22
Number in full: 1865
Average length of the domain: 173.10 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 55.71 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.7 20.7
Trusted cut-off 20.7 20.7
Noise cut-off 20.6 20.6
Model length: 200
Family (HMM) version: 12
Download: download the raw HMM for this family

Species distribution

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