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1  structure 107  species 0  interactions 287  sequences 6  architectures

Family: CAMSAP_CKK (PF08683)

Summary: Microtubule-binding calmodulin-regulated spectrin-associated

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This is the Wikipedia entry entitled "Calmodulin-regulated spectrin-associated CKK domain". More...

Calmodulin-regulated spectrin-associated CKK domain Edit Wikipedia article

CAMSAP_CKK
PDB 1ugj EBI.jpg
solution structure of a murine hypothetical protein from riken cdna 2310057j16
Identifiers
Symbol CAMSAP_CKK
Pfam PF08683
Pfam clan CL0350
InterPro IPR014797
SCOP 1ugj
SUPERFAMILY 1ugj

In molecular biology, the calmodulin-regulated spectrin-associated CKK domain (also known as the CKK domain) is a domain which occurs at the C-terminus of a family of eumetazoan proteins collectively defined as calmodulin-regulated spectrin-associated, or CAMSAP, proteins. CAMSAP proteins carry an N-terminal region that includes the CH domain, a central region including a predicted coiled-coil and this C-terminal. This domain is the part of the CAMSAP proteins that binds to microtubules. The domain appears to act by producing inhibition of neurite extension, probably by blocking microtubule function. CKK represents a domain that has evolved with the metazoa. The structure of a this domain in murine hypothetical protein has shown the domain to adopt a mainly beta barrel structure with an associated alpha-helical hairpin.[1]

References[edit]

  1. ^ Baines, A. J.; Bignone, P. A.; King, M. D. A.; Maggs, A. M.; Bennett, P. M.; Pinder, J. C.; Phillips, G. W. (2009). "The CKK Domain (DUF1781) Binds Microtubules and Defines the CAMSAP/ssp4 Family of Animal Proteins". Molecular Biology and Evolution 26 (9): 2005–2014. doi:10.1093/molbev/msp115. PMID 19508979.  edit

This article incorporates text from the public domain Pfam and InterPro IPR014797

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Microtubule-binding calmodulin-regulated spectrin-associated Provide feedback

This is the C-terminal domain of a family of eumetazoan proteins collectively defined as calmodulin-regulated spectrin-associated, or CAMSAP, proteins. CAMSAP proteins carry an N-terminal region that includes the CH domain, a central region including a predicted coiled-coil and this C-terminal, or CKK, domain - defined as being present in CAMSAP, KIAA1078 and KIAA1543, The C-terminal domain is the part of the CAMSAP proteins that binds to microtubules. The domain appears to act by producing inhibition of neurite extension, probably by blocking microtubule function. CKK represents a domain that has evolved with the metazoa. The structure of a murine hypothetical protein from RIKEN cDNA has shown the domain to adopt a mainly beta barrel structure with an associated alpha-helical hairpin.

Literature references

  1. Baines AJ, Bignone PA, King MD, Maggs AM, Bennett PM, Pinder JC, Phillips GW;, Mol Biol Evol. 2009 Jun 9. [Epub ahead of print]: The CKK domain (DUF1781) domain binds microtubules and defines the CAMSAP/ssp4 family of animal proteins. PUBMED:19508979 EPMC:19508979


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR014797

The CKK domain occurs at the C terminus of a family of proteins collectively defined as calmodulin-regulated spectrin-associated (or CAMSAP) proteins. CAMSAP proteins carry an N-terminal region that includes a CH domain, a central region including a predicted coiled-coil, and this C-terminal CKK domain which is involved in binding CAMSAP proteins to microtubules [PUBMED:19508979].

The structure of the CKK domain is a beta-barrel with an associated alpha-helical hairpin. Characteristically, the CKK domain has a single invariant tryptophan residue within the core of the predicted beta-barrel. Residues that interact with this Trp to form part of this core are highly conserved too.

Gene Ontology

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Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PRC-barrel (CL0350), which contains the following 3 members:

CAMSAP_CKK DUF2171 PRC

Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

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(14)
Full
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Representative proteomes NCBI
(255)
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RP35
(60)
RP55
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RP75
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  Seed
(14)
Full
(287)
Representative proteomes NCBI
(255)
Meta
(3)
RP15
(44)
RP35
(60)
RP55
(101)
RP75
(153)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(14)
Full
(287)
Representative proteomes NCBI
(255)
Meta
(3)
RP15
(44)
RP35
(60)
RP55
(101)
RP75
(153)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

Seed source: pdb_1ugj
Previous IDs: DUF1781; CKK;
Type: Domain
Author: Mistry J, Baines A
Number in seed: 14
Number in full: 287
Average length of the domain: 125.80 aa
Average identity of full alignment: 57 %
Average coverage of the sequence by the domain: 10.32 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 19.6 19.6
Trusted cut-off 20.3 22.3
Noise cut-off 19.0 18.3
Model length: 124
Family (HMM) version: 6
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CAMSAP_CKK domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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