Summary: KCNMB2, ball and chain domain

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Wikipedia: KCNMB2
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KCNMB2 Edit Wikipedia article

Potassium large conductance calcium-activated channel, subfamily M, beta member 2

PDB rendering based on 1jo6.

Available structures

PDB

Ortholog search: PDBe, RCSB

List of PDB id codes
1JO6

Identifiers

Symbols

KCNMB2; MGC22431

External IDs

OMIM: 605214 MGI: 1919663 HomoloGene: 4257 GeneCards: KCNMB2 Gene

Gene Ontology
Molecular function	â¢ ion channel inhibitor activity â¢ calcium-activated potassium channel activity â¢ potassium channel regulator activity
Cellular component	â¢ plasma membrane â¢ integral to plasma membrane â¢ voltage-gated potassium channel complex
Biological process	â¢ regulation of action potential â¢ detection of calcium ion â¢ potassium ion transport â¢ synaptic transmission â¢ blood coagulation â¢ regulation of action potential in neuron â¢ regulation of vasoconstriction
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 3:
177.99 â 178.56 Mb

Chr 3:
31.9 â 32.2 Mb

PubMed search

[1]

[2]

KCNMB2, ball and chain domain

solution structure of the cytoplasmic n-terminus of the bk beta-subunit kcnmb2

Identifiers

Symbol

KcnmB2_inactiv

Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe
PDBsum	structure summary

Calcium-activated potassium channel subunit beta-2 is a protein that in humans is encoded by the KCNMB2 gene.^[1]^[2]

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can contain two distinct subunits: a pore-forming alpha subunit and a modulatory beta subunit. Each complete MaxiK channel contains four copies of the pore-forming alpha subunit and up to four beta subunits. The protein encoded by the KCNMB2 gene is an auxiliary beta subunit which influences the calcium sensitivity of MaxiK currents and, following activation of MaxiK current, causes persistent inactivation. The subunit encoded by the KCNMB2 gene is expressed in various endocrine cells, including pancreas and adrenal chromaffin cells. It is also found in the brain, including the hippocampus. The KCNMB2 gene is homologous to three other genes found in mammalian genomes: KCNMB1 (found primarily in smooth muscle), KCNMB3, and KCNMB4 (the primary brain MaxiK auxiliary subunit).^[2]

Calcium-activated potassium channel subunit beta-2 comprises two domains. An N-terminal cytoplasmic domain, the ball and chain domain, which is responsible for the fast inactivation of these channels,^[3] and a C-terminal calcium-activated potassium channel beta subunit domain. The N-terminal domain only occurs in calcium-activated potassium channel subunit beta-2, while the C-terminal domain is found in related proteins.

[edit] See also

[edit] References

^ Wallner M, Meera P, Toro L (May 1999). "Molecular basis of fast inactivation in voltage and Ca2+-activated K+ channels: a transmembrane beta-subunit homolog". Proc Natl Acad Sci U S A 96 (7): 4137â42. doi:10.1073/pnas.96.7.4137. PMC 22433. PMID 10097176.
^ ^a ^b "Entrez Gene: KCNMB2 potassium large conductance calcium-activated channel, subfamily M, beta member 2".
^ Bentrop D, Beyermann M, Wissmann R, Fakler B (November 2001). "NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels". J. Biol. Chem. 276 (45): 42116â21. doi:10.1074/jbc.M107118200. PMID 11517232.

[edit] Further reading

Orio P, Rojas P, Ferreira G, Latorre R (2002). "New disguises for an old channel: MaxiK channel beta-subunits". News Physiol. Sci. 17: 156â61. PMID 12136044.
Xia XM, Ding JP, Lingle CJ (1999). "Molecular basis for the inactivation of Ca2+- and voltage-dependent BK channels in adrenal chromaffin cells and rat insulinoma tumor cells". J. Neurosci. 19 (13): 5255â64. PMID 10377337.
Brenner R, Jegla TJ, Wickenden A, et al. (2000). "Cloning and functional characterization of novel large conductance calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4". J. Biol. Chem. 275 (9): 6453â61. doi:10.1074/jbc.275.9.6453. PMID 10692449.
Liu QH, Williams DA, McManus C, et al. (2000). "HIV-1 gp120 and chemokines activate ion channels in primary macrophages through CCR5 and CXCR4 stimulation". Proc. Natl. Acad. Sci. U.S.A. 97 (9): 4832â7. doi:10.1073/pnas.090521697. PMC 18318. PMID 10758170.
Uebele VN, Lagrutta A, Wade T, et al. (2000). "Cloning and functional expression of two families of beta-subunits of the large conductance calcium-activated K+ channel". J. Biol. Chem. 275 (30): 23211â8. doi:10.1074/jbc.M910187199. PMID 10766764.
Meera P, Wallner M, Toro L (2000). "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin". Proc. Natl. Acad. Sci. U.S.A. 97 (10): 5562â7. doi:10.1073/pnas.100118597. PMC 25868. PMID 10792058.
Bentrop D, Beyermann M, Wissmann R, Fakler B (2001). "NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels". J. Biol. Chem. 276 (45): 42116â21. doi:10.1074/jbc.M107118200. PMID 11517232.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899â903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Xia XM, Ding JP, Lingle CJ (2003). "Inactivation of BK channels by the NH2 terminus of the beta2 auxiliary subunit: an essential role of a terminal peptide segment of three hydrophobic residues". J. Gen. Physiol. 121 (2): 125â48. doi:10.1085/jgp.20028667. PMC 2217327. PMID 12566540.
Hartness ME, Brazier SP, Peers C, et al. (2004). "Post-transcriptional control of human maxiK potassium channel activity and acute oxygen sensitivity by chronic hypoxia". J. Biol. Chem. 278 (51): 51422â32. doi:10.1074/jbc.M309463200. PMID 14522958.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121â7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Orio P, Torres Y, Rojas P, et al. (2006). "Structural determinants for functional coupling between the beta and alpha subunits in the Ca2+-activated K+ (BK) channel". J. Gen. Physiol. 127 (2): 191â204. doi:10.1085/jgp.200509370. PMC 2151488. PMID 16446507.
Zeng XH, Benzinger GR, Xia XM, Lingle CJ (2007). "BK channels with beta3a subunits generate use-dependent slow afterhyperpolarizing currents by an inactivation-coupled mechanism". J. Neurosci. 27 (17): 4707â15. doi:10.1523/JNEUROSCI.0758-07.2007. PMID 17460083.
Zarei MM, Song M, Wilson RJ, et al. (2007). "Endocytic trafficking signals in KCNMB2 regulate surface expression of a large conductance voltage and Ca(2+)-activated K+ channel". Neuroscience 147 (1): 80â9. doi:10.1016/j.neuroscience.2007.04.019. PMID 17521822.

[edit] External links

KCNMB2 human gene location in the UCSC Genome Browser.
KCNMB2 human gene details in the UCSC Genome Browser.

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This article incorporates text from the United States National Library of Medicine, which is in the public domain.

PDB gallery

1jo6: Solution structure of the cytoplasmic N-terminus of the BK beta-subunit KCNMB2

Membrane transport protein: ion channels (TC 1A)

Ca²⁺: Calcium channel

Ligand-gated	Inositol trisphosphate receptor (1, 2, 3) Â· Ryanodine receptor (1, 2, 3)

Voltage-gated	L-type/Ca_vÎ± (1.1, 1.2, 1.3, 1.4) Â· N-type/Ca_vÎ±2.2 Â· P-type/Ca_vÎ±(2.1) Â· Q-type/Ca_vÎ±2.1 Â· R-type/Ca_vÎ±2.3 Â· T-type/Ca_vÎ±(3.1, 3.2, 3.3) Î±₂Î´-subunits (1, 2) Â· Î²-subunits (Î²1, Î²2, Î²3, Î²4) Â· Î³-subunits (Î³1, Î³2, Î³3, Î³4) Cation channels of sperm (1, 2, 3, 4) Â· Two-pore channel (1, 2)

Na⁺: Sodium channel

Constitutively active	Epithelial sodium channel (Î±, Î², Î³, Î´)

Proton-gated	Amiloride-sensitive cation channel (1, 2, 3, 4)

Voltage-gated	Na_vÎ± (1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 7A) Â· Na_vÎ² (1, 2, 3, 4)

K⁺: Potassium channel

Calcium-activated	BK channel (Î±1, Î²1, Î²2, Î²3, Î²4) Â· SK channel (SK1, SK2, SK3) Â· IK channel (IK1) Â· K_Ca (1.1, 2.1, 2.2, 2.3, 3.1, 4.1, 4.2, 5.1)

Inward-rectifier	K_ATP Â· K_ir (1.1, 2.1, 2.2, 2.3, 2.4, 2.6) Â· GIRK/K_ir (3.1, 3.2, 3.3, 3.4) Â· K_ir (4.1, 4.2, 5.1, 6.1, 6.2, 7.1)

Tandem pore domain	K2P (1, 2, 3, 4, 5, 6, 7, 9, 10, 12, 13, 15, 16, 17, 18)

Voltage-gated	K_vÎ±1-6 (1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8) Â· (2.1, 2.2) Â· (3.1, 3.2, 3.3, 3.4) Â· (4.1, 4.2, 4.3) Â· (5.1) Â· (6.1, 6.2, 6.3, 6.4) K_vÎ±7-12 (7.1, 7.2, 7.3, 7.4, 7.5) Â· (8.1, 8.2) Â· (9.1, 9.2, 9.3) Â· (10.1, 10.2) Â· (11.1/hERG, 11.2, 11.3) Â· (12.1, 12.2, 12.3) K_vÎ² (1, 2, 3) Â· KCNIP (1, 2, 3, 4) Â· minK/ISK Â· minK/ISK-like Â· MiRP (1, 2, 3) Â· Shaker gene

Miscellaneous

Cl^-: Chloride channel	ANO1 Â· Bestrophin (1, 2) Â· CFTR Â· CLCA (1, 2, 3, 4) Â· CLCN (1, 2, 3, 4, 5, 6, 7, KA, KB) Â· CLIC (1, 2, 3, 4, 5, 6, L1) Â· CLNS (1A, 1B)

H⁺: Proton channel	HVCN1

M⁺: CNG cation channel	Î± (1, 2, 3, 4) Â· Î² (1, 2, 3) Â· HCN (FC, 1, 2, 3, 4)

M⁺: TRP cation channel	TRPA (1) Â· TRPC (1, 2, 3, 4, 4AP, 5, 6, 7) Â· TRPM (1, 2, 3, 4, 5, 6, 7, 8) Â· TRPML (1, 2, 3) Â· TRPN Â· TRPP (1, 2) Â· TRPV (1, 2, 3, 4, 5, 6)

H₂O (+ solutes): Porin	Aquaporin (0, 1, 2, 3, 4, 5, 6, 7, 8, 9) Â· Voltage-dependent anion channel (1, 2, 3) Â· General bacterial porin family

Cytoplasm: Gap junction	Connexin: A (GJA1, GJA3, GJA4, GJA5, GJA8, GJA9, GJA10) Â· B (GJB1, GJB2, GJB3, GJB4, GJB5, GJB6, GJB7) Â· C (GJC1, GJC2, GJC3) Â· D (GJD2, GJD3, GJD4)) Innexin

By gating mechanism

Ion channel class	Ligand-gated Â· Light-gated Â· Voltage-gated Â· Stretch-activated

see also disorders
B memb: cead, trns (1A, 1C, 1F, 2A, 3A1, 3A2-3, 3D), othr

This article incorporates text from the public domain Pfam and InterPro IPR015382

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

KCNMB2, ball and chain domain Provide feedback

Members of this family are found in the cytoplasmic N-terminus of KCNMB2, the beta-2 subunit of large conductance calcium and voltage-activated potassium channels. They are responsible for the fast inactivation of these channels [1].

Literature references

Bentrop D, Beyermann M, Wissmann R, Fakler B; , J Biol Chem. 2001;276:42116-42121.: NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels. PUBMED:11517232 EPMC:11517232

External database links

PANDIT:	PF09303
Pseudofam:	PF09303
SYSTERS:	KcnmB2_inactiv

This tab holds annotation information from the InterPro database.

InterPro entry IPR015382

This domain is found in the cytoplasmic N terminus of KCNMB2, the beta-2 subunit of large conductance calcium and voltage-activated potassium channels. It is responsible for the fast inactivation of these channels [PUBMED:11517232].

11517232

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

There are various ways to view or download the sequence alignments that we store. We provide several sequence viewers and a plain-text Stockholm-format file for download.

Alignment types

We make a range of alignments for each Pfam-A family:

seed: the curated alignment from which the HMM for the family is built
full: the alignment generated by searching the sequence database using the HMM
RP15/RP35/RP55/RP75: Representative Proteomes (RPs) at 15%, 35%, 55% and 75% co-membership thresholds
NCBI: alignment generated by searching the NCBI sequence database using the family HMM
meta: alignment generated by searching the metagenomics sequence database using the family HMM

Viewing

You can see the alignments as HTML or in three different sequence viewers:

jalview: a Java applet developed at the University of Dundee. You will need Java installed before running jalview
HTML: an HTML page showing the whole alignment.Please note: full Pfam alignments can be very large. These HTML views are extremely large and often cause problems for browsers. Please use either jalview or the Pfam viewer if you have trouble viewing the HTML version
PP/Heatmap: an HTML-based representation of the alignment, coloured according to the posterior-probability (PP) values from the HMM. As for the standard HTML view, heatmap alignments can also be very large and slow to render.
Pfam viewer: an HTML-based viewer that uses DAS to retrieve alignment fragments on request

Reformatting

You can download (or view in your browser) a text representation of a Pfam alignment in various formats:

Selex
Stockholm
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MSF

You can also change the order in which sequences are listed in the alignment, change how insertions are represented, alter the characters that are used to represent gaps in sequences and, finally, choose whether to download the alignment or to view it in your browser directly.

Downloading

You may find that large alignments cause problems for the viewers and the reformatting tool, so we also provide all alignments in Stockholm format. You can download either the plain text alignment, or a gzipped version of it.

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

	Seed (7)	Full (63)	Representative proteomes				NCBI (55)	Meta (0)
	Seed (7)	Full (63)	RP15 (1)	RP35 (3)	RP55 (9)	RP75 (25)	NCBI (55)	Meta (0)
Jalview	View	View	View	View	View	View	View
HTML	View	View	View	View	View	View
PP/heatmap	₁	View	View	View	View	View
Pfam viewer	View	View

¹Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: available, not generated, — not available.

Format an alignment

	Seed (7)	Full (63)	Representative proteomes				NCBI (55)	Meta (0)
	Seed (7)	Full (63)	RP15 (1)	RP35 (3)	RP55 (9)	RP75 (25)	NCBI (55)	Meta (0)
Alignment:
Format:
Order:	Tree Alphabetical
Sequence:	Inserts lower case All upper case
Gaps:
Download/view:	Download View

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

	Seed (7)	Full (63)	Representative proteomes				NCBI (55)	Meta (0)
	Seed (7)	Full (63)	RP15 (1)	RP35 (3)	RP55 (9)	RP75 (25)	NCBI (55)	Meta (0)
Raw Stockholm	Download	Download	Download	Download	Download	Download	Download
Gzipped	Download	Download	Download	Download	Download	Download	Download

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation

Seed source:

pdb_1jo6

Previous IDs:

none

Type:

Domain

Author:

Mistry J, Sammut SJ

Number in seed:

7

Number in full:

63

Average length of the domain:

29.60 aa

Average identity of full alignment:

80 %

Average coverage of the sequence by the domain:

15.20 %

HMM information

HMM build commands:

build method: hmmbuild -o /dev/null HMM SEED

search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq

Model details:

Parameter	Sequence	Domain
Gathering cut-off	25.0	25.0
Trusted cut-off	25.3	25.0
Noise cut-off	24.5	24.4

Model length:

32

Family (HMM) version:

5

Download:

download the raw HMM for this family

Species distribution

Sunburst
Tree

Sunburst controls

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

This chart is a modified "sunburst" visualisation of the species tree for this family. It shows each node in the tree as a separate arc, arranged radially with the superkingdoms at the centre and the species arrayed around the outermost ring.

How the sunburst is generated

The tree is built by considering the taxonomic lineage of each sequence that has a match to this family. For each node in the resulting tree, we draw an arc in the sunburst. The radius of the arc, its distance from the root node at the centre of the sunburst, shows the taxonomic level ("superkingdom", "kingdom", etc). The length of the arc represents either the number of sequences represented at a given level, or the number of species that are found beneath the node in the tree. The weighting scheme can be changed using the sunburst controls.

In order to reduce the complexity of the representation, we reduce the number of taxonomic levels that we show. We consider only the following eight major taxonomic levels:

superkingdom
kingdom
phylum
class
order
family
genus
species

Colouring and labels

Segments of the tree are coloured approximately according to their superkingdom. For example, archeal branches are coloured with shades of orange, eukaryotes in shades of purple, etc. The colour assignments are shown under the sunburst controls. Where space allows, the name of the taxonomic level will be written on the arc itself.

As you move your mouse across the sunburst, the current node will be highlighted. In the top section of the controls panel we show a summary of the lineage of the currently highlighed node. If you pause over an arc, a tooltip will be shown, giving the name of the taxonomic level in the title and a summary of the number of sequences and species below that node in the tree.

Anomalies in the taxonomy tree

There are some situations that the sunburst tree cannot easily handle and for which we have work-arounds in place.

Missing taxonomic levels

Some species in the taxonomic tree may not have one or more of the main eight levels that we display. For example, Bos taurus is not assigned an order in the NCBI taxonomic tree. In such cases we mark the omitted level with, for example, "No order", in both the tooltip and the lineage summary.

Unmapped species names

The tree is built by looking at each sequence in the full alignment for the family. We take the name of the species given by UniProt and try to map that to the full taxonomic tree from NCBI. In some cases, the name chosen by UniProt does not map to any node in the NCBI tree, perhaps because the chosen name is listed as a synonym or a misspelling in the NCBI taxonomy.

So that these nodes are not simply omitted from the sunburst tree, we group them together in a separate branch (or segment of the sunburst tree). Since we cannot determine the lineage for these unmapped species, we show all levels between the superkingdom and the species as "uncategorised".

Sub-species

Since we reduce the species tree to only the eight main taxonomic levels, sequences that are mapped to the sub-species level in the tree would not normally be shown. Rather than leave out these species, we map them instead to their parent species. So, for example, for sequences belonging to one of the Vibrio cholerae sub-species in the NCBI taxonomy, we show them instead as belonging to the species Vibrio cholerae.

Too many species/sequences

For large species trees, you may see blank regions in the outer layers of the sunburst. These occur when there are large numbers of arcs to be drawn in a small space. If an arc is less than approximately one pixel wide, it will not be drawn and the space will be left blank. You may still be able to get some information about the species in that region by moving your mouse across the area, but since each arc will be very small, it will be difficult to accurately locate a particular species.

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Tree controls

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The tree shows the occurrence of this domain across different species. More...

Species trees

We show the species tree in one of two ways. For smaller trees we try to show an interactive representation, which allows you to select specific nodes in the tree and view them as an alignment or as a set of Pfam domain graphics.

Unfortunately we have found that there are problems viewing the interactive tree when the it becomes larger than a certain limit. Furthermore, we have found that Internet Explorer can become unresponsive when viewing some trees, regardless of their size. We therefore show a text representation of the species tree when the size is above a certain limit or if you are using Internet Explorer to view the site.

If you are using IE you can still load the interactive tree by clicking the "Generate interactive tree" button, but please be aware of the potential problems that the interactive species tree can cause.

Interactive tree

For all of the domain matches in a full alignment, we count the number that are found on all sequences in the alignment. This total is shown in the purple box.

We also count the number of unique sequences on which each domain is found, which is shown in green. Note that a domain may appear multiple times on the same sequence, leading to the difference between these two numbers.

Finally, we group sequences from the same organism according to the NCBI code that is assigned by UniProt, allowing us to count the number of distinct sequences on which the domain is found. This value is shown in the pink boxes.

We use the NCBI species tree to group organisms according to their taxonomy and this forms the structure of the displayed tree. Note that in some cases the trees are too large (have too many nodes) to allow us to build an interactive tree, but in most cases you can still view the tree in a plain text, non-interactive representation. Those species which are represented in the seed alignment for this domain are highlighted.

You can use the tree controls to manipulate how the interactive tree is displayed:

show/hide the summary boxes
highlight species that are represented in the seed alignment
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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the KcnmB2_inactiv domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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Archea	Eukaryota
Bacteria	Other sequences
Viruses	Unclassified
Viroids	Unclassified sequence

Family: KcnmB2_inactiv (PF09303)

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