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0  structures 200  species 1  interaction 305  sequences 48  architectures

Family: CAF-1_p150 (PF11600)

Summary: Chromatin assembly factor 1 complex p150 subunit, N-terminal

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Chromatin assembly factor 1 complex p150 subunit, N-terminal Provide feedback

CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesised and acetylated histones H3/H4 into chromatin during DNA replication and repair [1]. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesised and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis [2].

Literature references

  1. Thiru A, Nietlispach D, Mott HR, Okuwaki M, Lyon D, Nielsen PR, Hirshberg M, Verreault A, Murzina NV, Laue ED; , EMBO J. 2004;23:489-499.: Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin. PUBMED:14765118 EPMC:14765118

  2. Reese BE, Bachman KE, Baylin SB, Rountree MR;, Mol Cell Biol. 2003;23:3226-3236.: The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. PUBMED:12697822 EPMC:12697822


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR021644

P150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesised and acetylated histones H3/H4 into chromatin during DNA replication and repair [PUBMED:14765118].P150 is the HP1 interaction site of CAF-1 and lies within the N-terminal region of the protein [PUBMED:14765118].

Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(13)
Full
(305)
Representative proteomes NCBI
(304)
Meta
(4)
RP15
(66)
RP35
(109)
RP55
(166)
RP75
(206)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(13)
Full
(305)
Representative proteomes NCBI
(304)
Meta
(4)
RP15
(66)
RP35
(109)
RP55
(166)
RP75
(206)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(13)
Full
(305)
Representative proteomes NCBI
(304)
Meta
(4)
RP15
(66)
RP35
(109)
RP55
(166)
RP75
(206)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: pdb_1s4z
Previous IDs: none
Type: Family
Author: Pollington J
Number in seed: 13
Number in full: 305
Average length of the domain: 187.60 aa
Average identity of full alignment: 19 %
Average coverage of the sequence by the domain: 26.44 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.4 22.4
Trusted cut-off 22.4 22.4
Noise cut-off 22.3 22.3
Model length: 216
Family (HMM) version: 3
Download: download the raw HMM for this family

Species distribution

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Interactions

There is 1 interaction for this family. More...

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