Summary: Pup-ligase protein
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Pup-ligase protein Provide feedback
Pupylation is a novel protein modification system found in some bacteria . This family of proteins are the enzyme that can conjugate proteins of the Pup family to lysine residues in target proteins marking them for degradation. The archetypal protein in this family is PafA (proteasome accessory factor) from Mycobacterium tuberculosis . It has been suggested that these proteins are related to gamma-glutamyl-cysteine synthetases .
Pearce MJ, Mintseris J, Ferreyra J, Gygi SP, Darwin KH;, Science. 2008;322:1104-1107.: Ubiquitin-like protein involved in the proteasome pathway of Mycobacterium tuberculosis. PUBMED:18832610 EPMC:18832610
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR004347
Pupylation is a novel protein modification system found in some bacteria [PUBMED:18980670]. This entry represents two related groups of proteins involved in this system. Pup ligases, such as PafA, conjugate the prokaryotic ubiquitin-like protein Pup to lysine residues in target proteins, marking them for degradation [PUBMED:18832610]. Pup deamidases, such as PafD, catalyse the deamidation of the Pup C-terminal glutamine to glutamate, thereby rendering the protein competent for conjugation [PUBMED:19448618]. It has been suggested that proteins in this entry are related to gamma-glutamyl-cysteine synthetases [PUBMED:18980670].
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|Biological process||modification-dependent protein catabolic process (GO:0019941)|
|proteasomal protein catabolic process (GO:0010498)|
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Curation and family details
|Seed source:||Pfam-B_3042 (release 6.5)|
|Previous IDs:||DUF245; Proteosome_20S;|
|Author:||Mifsud W, Bateman A|
|Number in seed:||31|
|Number in full:||731|
|Average length of the domain:||443.10 aa|
|Average identity of full alignment:||44 %|
|Average coverage of the sequence by the domain:||90.47 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||10|
|Download:||download the raw HMM for this family|
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