Summary: Type IV secretory system Conjugative DNA transfer
Type IV secretory system Conjugative DNA transfer Provide feedback
These proteins contain a P-loop and walker-B site for nucleotide binding. TraG is essential for DNA transfer in bacterial conjugation. These proteins are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems . The C-terminus of this domain interacts with the relaxosome component TraM via the latter's tetramerisation domain. TraD is a hexameric ring ATPase that forms the cytoplasmic face of the conjugative pore . The family contains a number of different DNA transfer proteins .
Dougherty BA, Hill C, Weidman JF, Richardson DR, Venter JC, Ross RP; , Mol Microbiol 1998;29:1029-1038.: Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147 PUBMED:9767571 EPMC:9767571
Schroder G, Krause S, Zechner EL, Traxler B, Yeo HJ, Lurz R, Waksman G, Lanka E; , J Bacteriol 2002;184:2767-2779.: TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates? PUBMED:11976307 EPMC:11976307
Lu J, Wong JJ, Edwards RA, Manchak J, Frost LS, Glover JN;, Mol Microbiol. 2008;70:89-99.: Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation. PUBMED:18717787 EPMC:18717787
Gunton JE, Gilmour MW, Baptista KP, Lawley TD, Taylor DE;, Microbiology. 2007;153:428-441.: Interaction between the co-inherited TraG coupling protein and the TraJ membrane-associated protein of the H-plasmid conjugative DNA transfer system resembles chromosomal DNA translocases. PUBMED:17259614 EPMC:17259614
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR003688
This entry represents TraG proteins and their homologues. These proteins contain a P-loop and walker-B site for nucleotide binding. TraG is essential for DNA transfer in bacterial conjugation. These proteins are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems [PUBMED:9767571, PUBMED:11976307].
|Cellular component||membrane (GO:0016020)|
|Biological process||unidirectional conjugation (GO:0009291)|
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Curation and family details
|Seed source:||Pfam-B_1146 (release 5.4)|
|Previous IDs:||TRAG; TraG;|
|Author:||Bashton M, Bateman A, Staddon J|
|Number in seed:||9|
|Number in full:||2504|
|Average length of the domain:||393.00 aa|
|Average identity of full alignment:||19 %|
|Average coverage of the sequence by the domain:||69.09 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||9|
|Download:||download the raw HMM for this family|
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