Summary: N2,N2-dimethylguanosine tRNA methyltransferase
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
N2,N2-dimethylguanosine tRNA methyltransferase Provide feedback
This enzyme EC:22.214.171.124 used S-AdoMet to methylate tRNA. The TRM1 gene of Saccharomyces cerevisiae is necessary for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNAs . The enzyme is found in both eukaryotes and archaebacteria 
Ellis SR, Hopper AK, Martin NC; , Proc Natl Acad Sci U S A 1987;84:5172-5176.: Amino-terminal extension generated from an upstream AUG codon is not required for mitochondrial import of yeast N2,N2-dimethylguanosine- specific tRNA methyltransferase. PUBMED:3299379 EPMC:3299379
Constantinesco F, Benachenhou N, Motorin Y, Grosjean H; , Nucleic Acids Res 1998;26:3753-3761.: The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli. PUBMED:9685492 EPMC:9685492
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR002905This enzyme EC dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups [PUBMED:9685492, PUBMED:10438627]. The TRM1 gene of Saccharomyces cerevisiae is required for the modification of both mitochondrial and cytoplasmic tRNAs [PUBMED:2426253].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||tRNA (guanine-N2-)-methyltransferase activity (GO:0004809)|
|RNA binding (GO:0003723)|
|Biological process||tRNA processing (GO:0008033)|
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|Seed source:||Enright A|
|Author:||Enright A, Ouzounis C, Bateman A|
|Number in seed:||10|
|Number in full:||846|
|Average length of the domain:||314.40 aa|
|Average identity of full alignment:||25 %|
|Average coverage of the sequence by the domain:||78.44 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||11|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the TRM domain has been found. There are 7 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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